Translator Disclaimer
1 January 2003 Evaluation of the Adhesive Capacity of Escherichia coli Isolates Associated with Avian Cellulitis
Benoît Leclerc, John M. Fairbrother, Martine Boulianne, Serge Messier
Author Affiliations +
Abstract

It has been shown that Escherichia coli isolates from lesions of cellulitis belong to a limited number of clonal groups distinct from those of isolates found in the environment of these birds. In this study, different in vitro methods were used to evaluate adherence properties of E. coli isolates from cellulitis lesions and environments of high- and low-cellulitis prevalence broiler flocks. One hundred isolates were tested by hemagglutination. Adherence to frozen sections of chicken skin and binding to soluble fibronectin were examined for 40 of these 100 isolates by immunofluorescence and by immunocytofluorometry, respectively. Localization of bacterial adherence to skin tissues was confirmed by immunohistochemistry. It was demonstrated that O78:K80 isolates from cellulitis lesions adhered to skin sections to a much greater extent in deeper than in superficial tissue layers. A greater bacterial adherence following growth in TSB at 37 C was demonstrated for isolates from flocks with high prevalence of cellulitis than for isolates from flocks with low prevalence of cellulitis. MANOVA analysis results showed a significant difference between superficial and deep tissue layers only for one set of isolates from flocks with high prevalence of cellulitis. Hemagglutinating activity was variable among the O78:K80 isolates obtained from flocks with high prevalence of cellulitis. The results obtained for some O78:K80 isolates following growth in TSB suggest a role for type 1 fimbriae or F1 in adherence to skin sections. This was reinforced by the finding that adherence was inhibited by d-mannose. Poultry E. coli isolates that express F1 had no affinity for soluble fibronectin, although localization of the adherence in skin sections suggested a role for extracellular matrix components such as collagen and insoluble fibronectin.

Benoît Leclerc, John M. Fairbrother, Martine Boulianne, and Serge Messier "Evaluation of the Adhesive Capacity of Escherichia coli Isolates Associated with Avian Cellulitis," Avian Diseases 47(1), 21-31, (1 January 2003). https://doi.org/10.1637/0005-2086(2003)047[0021:EOTACO]2.0.CO;2
Received: 25 September 2001; Published: 1 January 2003
JOURNAL ARTICLE
11 PAGES


Share
SHARE
KEYWORDS
adherence
cellulitis
Escherichia coli
F1 fimbriae
fibronectin
poultry
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top