Improved Polymerase Chain Reaction Technique for Determining the Species Composition of Eimeria in Poultry Litter

M. C. Jenkins; K. Miska; S. Klopp

doi:10.1637/7615-042106R.1

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1 December 2006 Improved Polymerase Chain Reaction Technique for Determining the Species Composition of Eimeria in Poultry Litter

M. C. Jenkins, K. Miska, S. Klopp

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Avian Diseases, 50(4):632-635 (2006). https://doi.org/10.1637/7615-042106R.1

Abstract

An improved polymerase chain reaction (PCR)-based method for determining the species composition of Eimeria in poultry litter was developed by incorporating species-specific internal standards in the assay. Internal standard molecules were prepared by fusing seven different Eimeria species-specific intervening transcribed sequence 1 (ITS1) rDNA primer pairs to a non-Eimeria DNA molecule and by cloning the hybrid DNA molecules into a plasmid. The internal DNA standards were then used in Eimeria-specific ITS1 PCR, and they were found to be capable of detecting E. acervulina, E. maxima, E. praecox, and E. tenella oocysts isolated directly from poultry litter.

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M. C. Jenkins, K. Miska, and S. Klopp "Improved Polymerase Chain Reaction Technique for Determining the Species Composition of Eimeria in Poultry Litter," Avian Diseases 50(4), 632-635, (1 December 2006). https://doi.org/10.1637/7615-042106R.1

Received: 21 April 2006; Accepted: 1 August 2006; Published: 1 December 2006

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