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1 December 2006 Improved Polymerase Chain Reaction Technique for Determining the Species Composition of Eimeria in Poultry Litter
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Abstract

An improved polymerase chain reaction (PCR)-based method for determining the species composition of Eimeria in poultry litter was developed by incorporating species-specific internal standards in the assay. Internal standard molecules were prepared by fusing seven different Eimeria species-specific intervening transcribed sequence 1 (ITS1) rDNA primer pairs to a non-Eimeria DNA molecule and by cloning the hybrid DNA molecules into a plasmid. The internal DNA standards were then used in Eimeria-specific ITS1 PCR, and they were found to be capable of detecting E. acervulina, E. maxima, E. praecox, and E. tenella oocysts isolated directly from poultry litter.

M. C. Jenkins, K. Miska, and S. Klopp "Improved Polymerase Chain Reaction Technique for Determining the Species Composition of Eimeria in Poultry Litter," Avian Diseases 50(4), 632-635, (1 December 2006). https://doi.org/10.1637/7615-042106R.1
Received: 21 April 2006; Accepted: 1 August 2006; Published: 1 December 2006
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