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1 December 2007 Replication and Transmission of Live Attenuated Infectious Laryngotracheitis Virus (ILTV) Vaccines
Andrés Rodríguez-Avila, Ivomar Oldoni, Sylva Riblet, Maricarmen García
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The aim of this study was to evaluate the replication of live attenuated infectious laryngotracheitis virus vaccines in selected tissues and their ability to transmit to contact-exposed birds. Four-week-old specific-pathogen-free chickens were eye drop–inoculated with tissue culture origin (TCO) and chicken embryo origin (CEO) vaccines. Contact-exposed chickens were housed in direct contact with eye drop–inoculated chickens from the first day postinoculation. Virus isolation and real-time polymerase chain reaction were used to detect the presence of live virus and viral DNA, respectively, in the trachea, trigeminal ganglia, eye conjunctiva, cecal tonsils, and cloaca from eye drop–inoculated and contact-exposed birds at days 2, 4, 5 to 10, 14, 18, 21, 24, and 28 postinoculation. No differences were observed in the ability of the TCO and CEO vaccines to replicate in the examined tissues. Both vaccines presented a localized replication in the eye conjunctiva and the trachea. Both vaccines were capable of transmitting to contact-exposed birds, attaining peaks of viral DNA as elevated as those observed in inoculated birds. The CEO vaccine replicated faster and reached higher viral genome copy number than the TCO vaccine in the conjunctiva and trachea of eye drop–inoculated and contact-exposed birds. The viral DNA from both vaccines migrated to the trigeminal ganglia during early stages of infection. Although the CEO and TCO vaccines were not recovered from the cecal tonsils and the cloaca, low levels of viral DNA were detected at these sites during the peak of viral replication in the upper respiratory tract.

Andrés Rodríguez-Avila, Ivomar Oldoni, Sylva Riblet, and Maricarmen García "Replication and Transmission of Live Attenuated Infectious Laryngotracheitis Virus (ILTV) Vaccines," Avian Diseases 51(4), 905-911, (1 December 2007).
Received: 20 April 2007; Accepted: 1 May 2007; Published: 1 December 2007
chicken embryo origin vaccine
genome copy number
infectious laryngotracheitis virus
tissue culture origin vaccine
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