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27 May 2019 Germ cell-less hybrid fish: ideal recipient for spermatogonial transplantation for the rapid production of donor-derived sperm
Dongdong Xu, Tasuku Yoshino, Junpei Konishi, Hiroyuki Yoshikawa, Yasuko Ino, Ryosuke Yazawa, Samyra Maria dos Santos Nassif Lacerda, Luiz Renato de França, Yutaka Takeuchi
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Abstract

An interspecific hybrid marine fish that developed a testis-like gonad without any germ cells, i.e., a germ cell-less gonad, was produced by hybridizing a female blue drum Nibea mitsukurii with a male white croaker Pennahia argentata. In this study, we evaluated the suitability of the germ cell-less fish as a recipient by transplanting donor testicular cells directly into the gonads through the urogenital papilla. The donor testicular cells were collected from hemizygous transgenic, green fluorescent protein (gfp) (+/–) blue drum, and transplanted into the germ cell-less gonads of the 6-month-old adult hybrid croakers. Fluorescent and histological observations showed the colonization, proliferation, and differentiation of transplanted spermatogonial cells in the gonads of hybrid croakers. The earliest production of spermatozoa in a hybrid recipient was observed at 7 weeks post-transplantation (pt), and 10% of the transplanted recipients produced donor-derived gfppositive spermatozoa by 25 weeks pt. Sperm from the hybrid recipients were used to fertilize eggs from wild-type blue drums, and approximately 50% of the resulting offspring were gfppositive, suggesting that all offspring originated from donor-derived sperm that were produced in the transplanted gfp (+/–) germ cells. To the best of our knowledge, this is the first report of successful spermatogonial transplantation using a germ cell-less adult fish as a recipient. This transplantation system has considerable advantages, such as the use of comparatively simple equipment and procedures, and rapid generation of donor-derived spermatogenesis and offspring, and presents numerous applications in commercial aquaculture.

Summary Sentence

Hybrid marine sciaenid fish showing germ cell-less phenotype are ideal recipients for developing spermatogonial transplantation in adult fish, and enable the rapid production of donor-derived sperm in 7 weeks post-transplantation.

© The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Dongdong Xu, Tasuku Yoshino, Junpei Konishi, Hiroyuki Yoshikawa, Yasuko Ino, Ryosuke Yazawa, Samyra Maria dos Santos Nassif Lacerda, Luiz Renato de França, and Yutaka Takeuchi "Germ cell-less hybrid fish: ideal recipient for spermatogonial transplantation for the rapid production of donor-derived sperm," Biology of Reproduction 101(2), 492-500, (27 May 2019). https://doi.org/10.1093/biolre/ioz045
Accepted: 24 May 2019; Published: 27 May 2019
JOURNAL ARTICLE
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KEYWORDS
fish reproduction
gametogenesis
Male infertility
spermatogenesis
spermatogonia
surrogate broodstock
testis
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