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22 July 2021 Hypoxia-induced alteration of RNA modifications in the mouse testis and sperm
Tong He, Huanping Guo, Xipeng Shen, Xiao Wu, Lin Xia, Xuelin Jiang, Yinying Xu, Dan Chen, Yunfang Zhang, Dongmei Tan, Yi Tan
Author Affiliations +
Abstract

Hypobaric hypoxia as an extreme environment in a plateau may have deleterious effects on human health. Studies have indicated that rush entry into a plateau may reduce male fertility and manifest in decreased sperm counts and weakened sperm motility. RNA modifications are sensitive to environmental changes and have recently emerged as novel post-transcriptional regulators in male spermatogenesis and intergenerational epigenetic inheritance. In the present study, we generated a mouse hypoxia model simulating the environment of 5500 m in altitude for 35 days, which led to compromised spermatogenesis, decreased sperm counts, and an increased sperm deformation rate. Using this hypoxia model, we further applied our recently developed high-throughput RNA modification quantification platform based on liquid chromatography with tandem mass spectrometry, which exhibited the capacity to simultaneously examine 25 types of RNA modifications. Our results revealed an altered sperm RNA modifications signature in the testis (6 types) and mature sperm (11 types) under the hypoxia model, with 4 types showing overlap (Am, Gm, m7G, and m22G). Our data first drew the signature of RNA modification profiles and comprehensively analyzed the alteration of RNA modification levels in mouse testis and sperm under a mouse hypoxia model. These data may be highly related to human conditions under a similar hypoxia environment.

Summary sentence

Using high-throughput LC–MS/MS, our study revealed an altered signature of RNA modifications in the mouse testis and sperm under a hypoxia model. These data may be strongly related to human conditions in a similar hypoxia environment.

Graphical Abstract

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© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Tong He, Huanping Guo, Xipeng Shen, Xiao Wu, Lin Xia, Xuelin Jiang, Yinying Xu, Dan Chen, Yunfang Zhang, Dongmei Tan, and Yi Tan "Hypoxia-induced alteration of RNA modifications in the mouse testis and sperm," Biology of Reproduction 105(5), 1171-1178, (22 July 2021). https://doi.org/10.1093/biolre/ioab142
Received: 23 February 2021; Accepted: 15 July 2021; Published: 22 July 2021
KEYWORDS
hypoxia
RNA modification
sperm
testis
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