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28 December 2021 The effects of vitrification on oocyte quality
Ching-Chien Chang, Daniel B. Shapiro, Zsolt Peter Nagy
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Vitrification, is an ultra-rapid, manual cooling process that produces glass-like (ice crystal-free) solidification. Water is prevented from forming intercellular and intracellular ice crystals during cooling as a result of oocyte dehydration and the use of highly concentrated cryoprotectant. Though oocytes can be cryopreserved without ice crystal formation through vitrification, it is still not clear whether the process of vitrification causes any negative impact (temperature change/chilling effect, osmotic stress, cryoprotectant toxicity, and/or phase transitions) on oocyte quality, which translates to diminished embryo developmental potential or subsequent clinical outcomes. In this review, we attempt to assess the technique's potential effects and the consequence of these effects on outcomes.

Summary Sentence: The osmolarity changes and water movement during oocyte vitrification and warming procedures are illustrated.

Graphical Abstract

© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail:
Ching-Chien Chang, Daniel B. Shapiro, and Zsolt Peter Nagy "The effects of vitrification on oocyte quality," Biology of Reproduction 106(2), 316-327, (28 December 2021).
Received: 31 August 2021; Accepted: 21 December 2021; Published: 28 December 2021
oocyte vitrification
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