How to translate text using browser tools
28 June 2022 Actions of WNT family member 5A to regulate characteristics of development of the bovine preimplantation embryo
Surawich Jeensuk, M. Sofia Ortega, Muhammad Saleem, Briana Hawryluk, Tracy L. Scheffler, Peter J. Hansen
Author Affiliations +
Abstract

WNT signaling is important for regulation of embryonic development. The most abundant WNT gene expressed in the bovine endometrium during the preimplantation period is WNT5A. One objective was to determine whether WNT5A regulates competence of the bovine preimplantation embryo to become a blastocyst and alters the number of cells in the inner cell mass and trophectoderm. A second objective was to delineate features of the cell-signaling mechanisms involved in WNT5A actions. WNT5A caused a concentration-dependent increase in the proportion of embryos developing to the blastocyst stage and in the number of inner cell mass cells in the resultant blastocysts. A concentration of 200 ng/mL was most effective, and a higher concentration of 400 ng/mL was not stimulatory. Bovine serum albumin in culture reduced the magnitude of effects of WNT5A on development to the blastocyst stage. WNT5A affected expression of 173 genes at the morula stage; all were upregulated by WNT5A. Many of the upregulated genes were associated with cell signaling. Actions of WNT5A on development to the blastocyst stage were suppressed by a Rho-associated coiled-coil kinase (ROCK) signaling inhibitor, suggesting that WNT5A acts through Ras homology gene family member A (RhoA)/ROCK signaling. Other experiments indicated that actions of WNT5A are independent of the canonical β-catenin signaling pathway and RAC1/c-Jun N-terminal kinase (JNK) signaling. This is the first report outlining the actions of WNT5A to alter the development of the mammalian embryo. These findings provide insights into how embryokines regulate maternal–embryonic communication.

Summary Sentence:

WNT5A, one of the most abundant cell-signaling ligands expressed in the endometrium, is an important regulator of early development because it can act on the preimplantation bovine embryo to improve competence to develop to the blastocyst stage and increase the number of cells in inner cell mass.

Graphical Abstract

img-z2-2_928.jpg
© The Author(s) 2022. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Surawich Jeensuk, M. Sofia Ortega, Muhammad Saleem, Briana Hawryluk, Tracy L. Scheffler, and Peter J. Hansen "Actions of WNT family member 5A to regulate characteristics of development of the bovine preimplantation embryo," Biology of Reproduction 107(4), 928-944, (28 June 2022). https://doi.org/10.1093/biolre/ioac127
Received: 2 March 2022; Accepted: 14 June 2022; Published: 28 June 2022
KEYWORDS
blastocyst
bovine
embryonic development
WNT
Wnt5a
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top