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30 June 2022 G protein-coupled estrogen receptor promotes acrosome reaction via regulation of Ca2+ signaling in mouse sperm
Dong-Dong Gao, Chong-Feng Lan, Xiao-Nian Cao, Lei Chen, Tian-Lun Lei, Lei Peng, Jia-Wen Xu, Zhuo-Er Qiu, Long-Long Wang, Qing Sun, Zi-Yang Huang, Yun-Xin Zhu, Wen-Liang Zhou, Yi-Lin Zhang
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Abstract

G protein-coupled estrogen receptor (GPER), a seven-transmembrane G protein-coupled receptor, mediates the rapid pre-genomic signaling actions of estrogen and derivatives thereof. The expression of GPER is extensive in mammal male reproductive system. However, the functional role of GPER in mouse sperm has not yet been well recognized. This study revealed that GPER was expressed at the acrosome and the mid-flagellum of the mouse sperm. The endogenous GPER ligand 17β-estradiol and the selective GPER agonist G1 increased intracellular Ca2+ concentration ([Ca2+]i) in mouse sperm, which could be abolished by G15, an antagonist of GPER. In addition, the G1-stimulated Ca2+ response was attenuated by interference with the phospholipase C (PLC) signaling pathways or by blocking the cation channel of sperm (CatSper). Chlortetracycline staining assay showed that the activation of GPER increased the incidence of acrosome-reacted sperm. Conclusively, GPER was located at the acrosome and mid-flagellum of the mouse sperm. Activation of GPER triggered the elevation of [Ca2+]i through PLC-dependent Ca2+ mobilization and CatSper-mediated Ca2+ influx, which promoted the acrosome reaction of mouse sperm.

Graphical Abstract

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© The Author(s) 2022. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Dong-Dong Gao, Chong-Feng Lan, Xiao-Nian Cao, Lei Chen, Tian-Lun Lei, Lei Peng, Jia-Wen Xu, Zhuo-Er Qiu, Long-Long Wang, Qing Sun, Zi-Yang Huang, Yun-Xin Zhu, Wen-Liang Zhou, and Yi-Lin Zhang "G protein-coupled estrogen receptor promotes acrosome reaction via regulation of Ca2+ signaling in mouse sperm," Biology of Reproduction 107(4), 1026-1034, (30 June 2022). https://doi.org/10.1093/biolre/ioac136
Received: 28 January 2022; Accepted: 22 June 2022; Published: 30 June 2022
KEYWORDS
acrosome reaction
Ca2+ signaling
CatSper
Gper
IP3R
PLC
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