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14 July 2022 Notch effector recombination signal binding protein for immunoglobulin kappa J signaling is required for the initiation of endometrial stromal cell decidualization
Genna E. Moldovan, Yong Song, Tae Hoon Kim, Ren-Wei Su, Jae-Wook Jeong, Artiom Gruzdev, Thomas E. Spencer, Asgerally T. Fazleabas
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Abstract

The Notch signaling pathway is required for reproductive success. This pathway activates its transcriptional effector, recombination signal binding protein for immunoglobulin kappa J (Rbpj), to induce transcription of its target genes. This signaling pathway is required for successful decidualization, implantation, and uterine repair following parturition. To identify the compartmental specific roles of the Notch signaling pathway in the establishment of pregnancy, we generated epithelial and decidual stromal cell specific knockouts of Rbpj utilizing lactoferrin iCre and Prl8A2 iCre, respectively. Both conditional knockout mouse models were fertile. The Rbpj epithelial knockout mice displayed 27% resorption sites at E15.5, but this did not significantly impact the number of live born pups compared with controls. In addition, the Rbpj epithelial knockout mice displayed increased estrogen signaling in their stromal compartment. Given that both mouse models exhibited fertility comparable to control animals, the epithelial and stromal specific nature of the iCre recombinases utilized, and previously published Rbpj total uterine knockout mouse models, we conclude that Notch effector Rbpj signaling is required at the initiation of pregnancy to support decidualization in stromal cells, but that Rbpj is not required in the epithelial compartment nor is it required for post-implantation pregnancy success.

Summary Sentence

Notch effector Rbpj signaling is required at the initiation of pregnancy to support decidualization in stromal cells, but Rbpj is not required in the epithelial compartment nor is it required for post-implantation pregnancy success.

© The Author(s) 2022. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Genna E. Moldovan, Yong Song, Tae Hoon Kim, Ren-Wei Su, Jae-Wook Jeong, Artiom Gruzdev, Thomas E. Spencer, and Asgerally T. Fazleabas "Notch effector recombination signal binding protein for immunoglobulin kappa J signaling is required for the initiation of endometrial stromal cell decidualization," Biology of Reproduction 107(4), 977-983, (14 July 2022). https://doi.org/10.1093/biolre/ioac140
Received: 5 April 2022; Accepted: 6 July 2022; Published: 14 July 2022
KEYWORDS
decidualization
implantation
Notch signaling
pregnancy
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