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14 July 2022 Ovine conceptus tissue metabolizes fructose for metabolic support during the peri-implantation period of pregnancy
Robyn M. Moses, Katherine M. Halloran, Claire Stenhouse, Nirvay Sah, Avery C. Kramer, Bryan A. McLendon, Heewon Seo, Gregory A. Johnson, Guoyao Wu, Fuller W. Bazer
Author Affiliations +
Abstract

Roles of fructose in elongating ovine conceptuses are poorly understood, despite it being the major hexose sugar in fetal fluids and plasma throughout gestation. Therefore, we determined if elongating ovine conceptuses utilize fructose via metabolic pathways for survival and development. Immunohistochemical analyses revealed that trophectoderm and extra-embryonic endoderm express ketohexokinase and aldolase B during the peri-implantation period of pregnancy for conversion of fructose into fructose-1-phosphate for entry into glycolysis and related metabolic pathways. Conceptus homogenates were cultured with 14C-labeled glucose and/or fructose under oxygenated and hypoxic conditions to assess contributions of glucose and fructose to the pentose cycle (PC), tricarboxylic acid cycle, glycoproteins, and lipid synthesis. Results indicated that both glucose and fructose contributed carbons to each of these pathways, except for lipid synthesis, and metabolized to pyruvate and lactate, with lactate being the primary product of glycolysis under oxygenated and hypoxic conditions. We also found that (1) conceptuses preferentially oxidized glucose over fructose (P < 0.05); (2) incorporation of fructose and glucose at 4 mM each into the PC by Day 16 conceptus homogenates was similar in the presence or absence of glucose, but incorporation of glucose into the PC was enhanced by the presence of fructose (P < 0.05); (3) incorporation of fructose into the PC in the absence of glucose was greater under oxygenated conditions (P < 0.01); and (4) incorporation of glucose into the PC under oxygenated conditions was greater in the presence of fructose (P = 0.05). These results indicate that fructose is an important metabolic substrate for ovine conceptuses.

Summary Sentence

Fructose and glucose are substrates for the pentose cycle and tricarboxylic acid cycle in ovine conceptuses.

© The Author(s) 2022. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Robyn M. Moses, Katherine M. Halloran, Claire Stenhouse, Nirvay Sah, Avery C. Kramer, Bryan A. McLendon, Heewon Seo, Gregory A. Johnson, Guoyao Wu, and Fuller W. Bazer "Ovine conceptus tissue metabolizes fructose for metabolic support during the peri-implantation period of pregnancy," Biology of Reproduction 107(4), 1084-1096, (14 July 2022). https://doi.org/10.1093/biolre/ioac144
Received: 5 April 2022; Accepted: 6 July 2022; Published: 14 July 2022
KEYWORDS
conceptus
fructose
Glucose
metabolism
ovine
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