Translator Disclaimer
1 September 2000 Expression of Cyclooxygenase 2 by Prostaglandin E2 in Human Endometrial Adenocarcinoma Cell Line HEC-1B
Iqbal Munir, Kohji Fukunaga, Haruhiko Kanasaki, Kohji Miyazaki, Takashi Ohba, Hitoshi Okamura, Eishichi Miyamoto
Author Affiliations +
Abstract

The regulation of expression of cyclooxygenase 2 (COX-2) was investigated by treatment with PGE2 in human endometrial adenocarcinoma cell line HEC-1B. One μM PGE2 could stimulate the expression of COX-2 approximately twofold in this cell line. The same concentration of PGE2 also stimulated activation of mitogen-activated protein kinase (MAP kinase) and protein kinase B (PKB). PGE2-induced MAP kinase activation was sensitive to a MAP kinase kinase (MEK) inhibitor, PD098059, and a protein kinase A inhibitor, H-89. PD098059 and H-89 also partially inhibited the expression of COX-2 stimulated by PGE2. PGE2 could stimulate the activation of PKB, which was sensitive to phosphatidylinositol-3-OH kinase (PI3K) inhibitor, wortmannin. Whereas wortmannin alone partially inhibited the expression of COX-2, a combination of wortmannin and PD098059 totally inhibited PGE2-mediated COX-2 expression. These results suggest that MAP kinase and PI3K pathways are stimulated with PGE2, and that both of these pathways are involved in the expression of COX-2. In addition, they also suggest that protein kinase A remains upstream of PGE2-induced activation of MAP kinase in HEC-1B cells.

Iqbal Munir, Kohji Fukunaga, Haruhiko Kanasaki, Kohji Miyazaki, Takashi Ohba, Hitoshi Okamura, and Eishichi Miyamoto "Expression of Cyclooxygenase 2 by Prostaglandin E2 in Human Endometrial Adenocarcinoma Cell Line HEC-1B," Biology of Reproduction 63(3), 933-941, (1 September 2000). https://doi.org/10.1095/biolreprod63.3.933
Received: 27 July 1999; Accepted: 1 May 2000; Published: 1 September 2000
JOURNAL ARTICLE
9 PAGES


Share
SHARE
KEYWORDS
kinases
signal transducers
signal transduction
uterus
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top