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1 September 2000 Fibroblast Growth Factor-10: A Stromal Mediator of Epithelial Function in the Ovine Uterus
Chen Chen, Thomas E. Spencer, Fuller W. Bazer
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Fibroblast growth factor-10 (FGF-10) is a stromal-derived paracrine growth factor considered to be important during embryogenesis; however, its expression by cells in the female reproductive tract has not been investigated. Therefore, an ovine FGF-10 cDNA was cloned from an ovine endometrial cDNA library to investigate expression and potential paracrine characteristics of FGF-10 in the ovine uterus. The ovine FGF-10 cDNA encodes a protein of 213 amino acids and possesses an unusually long 5′ untranslated region (UTR). In situ hybridization demonstrated that ovine FGF-10 mRNA was expressed by endometrial stromal cells and by mesenchymal cells of the chorioallantoic placenta. The mRNA for FGF-7, a homologue of FGF-10, was localized in the tunica muscularis of blood vessels in endometrium and myometrium. In contrast, FGF receptor 2IIIb, the high-affinity receptor for both FGF-10 and FGF-7, was expressed exclusively in luminal epithelium, glandular epithelium, and placental trophectoderm. The in vivo spatial expression pattern suggests that FGF-10 is a novel endometrial stromal cell-derived mediator of uterine epithelial and conceptus trophectodermal functions. The nonoverlapping spatial patterns of expression for FGF-10 and FGF-7 in ovine uterus and conceptus suggest independent roles in uterine function and conceptus development.

Chen Chen, Thomas E. Spencer, and Fuller W. Bazer "Fibroblast Growth Factor-10: A Stromal Mediator of Epithelial Function in the Ovine Uterus," Biology of Reproduction 63(3), 959-966, (1 September 2000).
Received: 21 October 1999; Accepted: 1 May 2000; Published: 1 September 2000

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