Translator Disclaimer
1 September 2001 Role of Tumor Necrosis Factor in Preovulatory Follicles of Swine
Janine Prange-Kiel, Claudia Kreutzkamm, Uwe Wehrenberg, Gabriele M. Rune
Author Affiliations +
Abstract

The effects of tumor necrosis factor (TNF) on cultured porcine granulosa cells that were obtained from preovulatory follicles were studied with regard to following parameters: 1) TNF receptor type I expression, 2) progesterone receptor and transforming growth factor β receptor type II (TβR II) as markers of luteinization, 3) proliferation, and 4) apoptosis. For comparative purposes the effects of TNF were also studied on insulin/forskolin-treated cells, as this treatment is well established to induce luteinization. Cytochemical methods followed by semiquantitative analysis were used. Our data show that TNF treatment upregulates TNF receptor type I expression in granulosa cells. TNF downregulates the expression of TβR II of insulin/forskolin-stimulated and of unstimulated cells. The progesterone receptor is also downregulated by the cytokine after insulin/forskolin-induced luteinization. Supplementation of the medium with TNF leads to increased proliferation and at the same time it induces apoptosis. Our results indicate that TNF exerts an inhibitory influence on luteinization and that TNF influences the balance between follicular growth (proliferation) and atresia (apoptosis).

Janine Prange-Kiel, Claudia Kreutzkamm, Uwe Wehrenberg, and Gabriele M. Rune "Role of Tumor Necrosis Factor in Preovulatory Follicles of Swine," Biology of Reproduction 65(3), 928-935, (1 September 2001). https://doi.org/10.1095/biolreprod65.3.928
Received: 3 January 2001; Accepted: 1 May 2001; Published: 1 September 2001
JOURNAL ARTICLE
8 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top