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1 February 2003 Signal Transduction Pathways Activated by Chorionic Gonadotropin in the Primate Endometrial Epithelial Cells
Santha Srisuparp, Zuzana Strakova, Allison Brudney, Sutapa Mukherjee, Scott Reierstad, Mary Hunzicker-Dunn, Asgerally T. Fazleabas
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Abstract

Successful implantation requires synergism between the developing embryo and the receptive endometrium. In the baboon, infusion of chorionic gonadotropin (CG) modulates both morphology and physiology of the epithelial and stromal cells of the receptive endometrium. This study explored the signal transduction pathways activated by CG in endometrial epithelial cells from baboon (BE) and human (HES). Incubations of BE and HES cells with CG did not significantly alter adenylyl cyclase activity or increase intracellular cAMP when compared with Chinese hamster ovarian cells stably transfected with the full-length human CG/luteinizing hormone (LH) receptor (CHO-LH cells). However, in BE and HES cells, CG induced the phosphorylation of several proteins, among them, extracellular signal-regulated protein kinases 1 and 2 (ERK 1/2). Phosphorylation of ERK 1/2 in uterine epithelial cells was protein kinase A (PKA) independent. This novel signaling pathway is functional because, in response to CG stimulation, prostaglandin E2 (PGE2) was released into the media and increased significantly 2 h following CG stimulation. CG-stimulated PGE2 synthesis in epithelial cells was inhibited by a specific mitogen-activated protein kinase (MEK 1/2) inhibitor, PD 98059. In conclusion, immediate signal transduction pathways induced by CG in endometrial epithelial cells are cAMP independent and stimulate phosphorylation of ERK 1/2 via a MEK 1/2 pathway, leading to an increase in PGE2 release as the possible result of cyclooxygenase-2 activation.

Santha Srisuparp, Zuzana Strakova, Allison Brudney, Sutapa Mukherjee, Scott Reierstad, Mary Hunzicker-Dunn, and Asgerally T. Fazleabas "Signal Transduction Pathways Activated by Chorionic Gonadotropin in the Primate Endometrial Epithelial Cells," Biology of Reproduction 68(2), 457-464, (1 February 2003). https://doi.org/10.1095/biolreprod.102.007625
Received: 27 May 2002; Accepted: 1 August 2002; Published: 1 February 2003
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KEYWORDS
human chorionic gonadotropin
signal transduction
uterus
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