Translator Disclaimer
1 November 2003 Requirement of Gap Junctional Intercellular Communication for Human Villous Trophoblast Differentiation
Laurent Cronier, Jean-Louis Frendo, Norah Defamie, Guillaume Pidoux, Gladys Bertin, Jean Guibourdenche, Georges Pointis, André Malassiné
Author Affiliations +
Abstract

During pregnancy, the villous trophoblast develops from the fusion of cytotrophoblastic cells (CT) into a syncytiotrophoblast (ST), supporting the main physiological functions of the human placenta. Connexin43 (Cx43) is demonstrated in situ and in vitro in the villous trophoblast between CT and between CT and ST. Moreover, the presence of a gap junctional intercellular communication (GJIC) during in vitro trophoblast differentiation was previously demonstrated. Because the exchange of molecules through gap junctions is considered to play a major role in the control of cell and tissue differentiation, we studied the effects of a gap junctional uncoupler, heptanol, on morphological and functional trophoblast differentiation and on GJIC measured by the fluorescence recovery after photobleaching method. We found that when the GJIC was interrupted, CT still aggregated but fused poorly. This morphological effect was associated with a significant decrease of trophoblastic-specific gene expression (β human chorionic gonadotropin and human chorionic somatomammotropin). This blocking action was reversible as demonstrated by recovery of GJIC and trophoblast differentiation process after heptanol removal. Moreover, the inhibition of the trophoblast differentiation did not affect Cx43 transcript expression and Cx43 protein expression. These data suggest that the molecular exchanges through gap junctions preceding cellular fusion are essential for trophoblast differentiation generating the multifunctional syncytiotrophoblast.

Laurent Cronier, Jean-Louis Frendo, Norah Defamie, Guillaume Pidoux, Gladys Bertin, Jean Guibourdenche, Georges Pointis, and André Malassiné "Requirement of Gap Junctional Intercellular Communication for Human Villous Trophoblast Differentiation," Biology of Reproduction 69(5), 1472-1480, (1 November 2003). https://doi.org/10.1095/biolreprod.103.016360
Received: 13 February 2003; Accepted: 1 June 2003; Published: 1 November 2003
JOURNAL ARTICLE
9 PAGES


Share
SHARE
KEYWORDS
human chorionic gonadotropin
placenta
signal transduction
syncytiotrophoblast
trophoblast
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top