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1 November 2003 Maturation and Fertilization of Porcine Oocytes from Primordial Follicles by a Combination of Xenografting and In Vitro Culture
Hiroyuki Kaneko, Kazuhiro Kikuchi, Junko Noguchi, Misa Hosoe, Tomiji Akita
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Abstract

Our objective was to develop a method of endowing oocytes from porcine primordial follicles with full maturation and fertilizing ability as a model for ovarian xenografting of large mammals. Ovarian tissues from 20-day-old piglets, in which most of the follicles were primordial, were transplanted under the capsules of both kidneys of ovariectomized athymic mice. The host mice were treated with 5 IU of equine chorionic gonadotropin (eCG) for 10 days (eCG-10), 30 days (eCG-30), or 60 days (eCG-60) after detection of cornified epithelial cells in their vaginal smears. Cumulus-oocyte complexes, ovarian grafts, and blood samples were obtained 48 h after eCG treatment. Forty-five to 70 days after grafting, the host mice in all groups for the first time showed vaginal cornification, accompanied by the formation of a small number of antral follicles in the grafts. However, we recovered large numbers of full-sized oocytes only from mice in the eCG-60 group; the numbers of full-sized oocytes in the other groups were low. Peripheral levels of total inhibin were highest in the eCG-60 group; this supports our finding that the most enhanced growth of antral follicles occurred in this eCG-60 group. Of 573 oocytes obtained from the eCG-60 group, 98 (17%) were at the metaphase II stage after in vitro culture for maturation. Moreover, 55% of matured oocytes with the first polar body (n = 20) were fertilized in vitro. These results clearly demonstrate that fertilization of oocytes from porcine primordial follicles is achievable by a combination of xenografting and in vitro culture.

Hiroyuki Kaneko, Kazuhiro Kikuchi, Junko Noguchi, Misa Hosoe, and Tomiji Akita "Maturation and Fertilization of Porcine Oocytes from Primordial Follicles by a Combination of Xenografting and In Vitro Culture," Biology of Reproduction 69(5), 1488-1493, (1 November 2003). https://doi.org/10.1095/biolreprod.103.017038
Received: 13 March 2003; Accepted: 1 June 2003; Published: 1 November 2003
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fertilization
follicular development
oocyte development
ovary
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