Translator Disclaimer
1 November 2003 Isolation of Differentially Expressed Genes in Conceptuses and Endometrial Tissue of Sows in Early Gestation
Maud Vallée, Danièle Beaudry, Claude Roberge, J. Jacques Matte, Richard Blouin, Marie-France Palin
Author Affiliations +
Abstract

The implantation period is a critical time for embryonic survival in pigs. During this period, numerous growth factors are secreted by the conceptuses and the uterine endometrium in order to establish pregnancy and to provide a proper environment for embryonic development. It is well known that the Chinese Meishan sows have a larger litter size when compared with occidental sows mainly because of a superior embryonic survival rate. As a further step toward understanding the mechanisms involved in embryonic survival, we used a suppression subtractive hybridization technique to identify genes that were differentially expressed in Meishan-Landrace conceptuses and endometrial tissue at Day 15 of gestation when compared with conventional Landrace sows. Of the 1000 subtractive clones isolated from each library, 137 endometrial and 166 conceptus-enriched cDNAs were single-pass sequenced and examined by BLAST analysis for identification. Sixty-two percent of the clones found in the endometrial library and 78% of the clones found in the conceptus library showed homology with known genes. Among these genes, the 20 most relevant to embryonic survival based on the available literature were validated through real-time reverse-transcriptase polymerase chain reaction (RT-PCR) analysis. Our results show that suppression subtractive hybridization is a powerful method applicable in identifying putative candidate genes that might be used for selection of high litter-size breeds.

Maud Vallée, Danièle Beaudry, Claude Roberge, J. Jacques Matte, Richard Blouin, and Marie-France Palin "Isolation of Differentially Expressed Genes in Conceptuses and Endometrial Tissue of Sows in Early Gestation," Biology of Reproduction 69(5), 1697-1706, (1 November 2003). https://doi.org/10.1095/biolreprod.103.019307
Received: 12 May 2003; Accepted: 1 July 2003; Published: 1 November 2003
JOURNAL ARTICLE
10 PAGES


Share
SHARE
KEYWORDS
conceptus
early development
implantation
uterus
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top