Tolerance of the Mouse Sperm Nuclei to Freeze-Drying Depends on Their Disulfide Status1

Takehito Kaneko; David G. Whittingham; James W. Overstreet; Ryuzo Yanagimachi

doi:10.1095/biolreprod.103.019729

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Takehito Kaneko,^1,2,* David G. Whittingham,¹ James W. Overstreet,³ Ryuzo Yanagimachi¹

¹aInstitute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A. Burns Sc
²bDivision of Reproductive Engineering, Center for Animal Resources and Development, Kumamoto Univers
³cDivision of Reproductive Biology, Department of Obstetrics and Gynecology, University of California

^*Correspondence: Takehito Kaneko, Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Road, Honolulu, HI 96822. FAX: 808 956 7316; takehito@hawaii.edu

Abstract

Mouse spermatozoa from the caudae epididymides could be freeze-dried without losing their ability to support normal development. Immature spermatozoa from the testes, in contrast, were damaged by freeze-drying. However, immature spermatozoa became resistant to freeze-drying after their treatment with diamide, which oxidizes free -SH groups. Conversely, epididymal spermatozoa were damaged by freeze-drying if first treated with dithiothreitol (DTT), which reduces -SS- bonds. The potential for freeze-drying damage seems likely to relate to the -SS- status of sperm proteins, in particular its protamines.

Citation Download Citation

Takehito Kaneko, David G. Whittingham, James W. Overstreet, and Ryuzo Yanagimachi "Tolerance of the Mouse Sperm Nuclei to Freeze-Drying Depends on Their Disulfide Status," Biology of Reproduction 69(6), 1859-1862, (1 December 2003). https://doi.org/10.1095/biolreprod.103.019729

Received: 26 May 2003; Accepted: 1 July 2003; Published: 1 December 2003

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