Translator Disclaimer
1 July 2004 Interactions Between Androgen and Growth Factors in Granulosa Cell Subtypes of Porcine Antral Follicles
T. E. Hickey, D. L. Marrocco, R. B. Gilchrist, R. J. Norman, D. T. Armstrong
Author Affiliations +
Abstract

Androgens acting via the androgen receptor (AR) have been implicated in regulation of folliculogenesis in many animal species. These effects are possibly mediated via enhancement of FSH and/or insulin-like growth factor (IGF)-I activity in granulosa cells, which contain high levels of AR protein. We examined the in vitro effect of dihydrotestosterone (DHT) on DNA synthesis and progesterone secretion by follicular cells in response to FSH and IGF-I, alone or in combination. Cells from separate pools of 1- to 3-mm and 3- to 5-mm antral follicles were aspirated from gilt ovaries and fractioned into mural granulosa cells (MGCs) and cumulus-oocyte complexes (COCs) for subsequent cell culture. Androgen alone or with any combination of mitogen had minimal effect on proliferative and no effect on steroidogenic responses of MGCs from 3- to 5-mm antral follicles. Conversely, in MGCs from 1- to 3-mm follicles, DHT significantly enhanced IFG-I-stimulated proliferation and had variable influence on progesterone secretion. The effects of DHT on proliferative responses of COCs were also dependent on follicle size: DHT significantly augmented either IGF-I-stimulated proliferation (1- to 3-mm follicles) or FSH-stimulated proliferation (3- to 5-mm follicles). However, the steroidogenic responses of all COCs were identical, whereby DHT significantly suppressed progesterone secretion, predominantly in the presence of FSH. Addition of an AR antagonist, hydroxyflutamide, generally reversed the proliferative responses invoked by DHT but not the steroidogenic responses. We conclude that androgen-receptor-mediated activity in granulosa cells of antral follicles is dependent on follicle size, is influenced by proximity of cells to the oocyte, and possibly involves both classic and nonclassic steroid mechanisms.

T. E. Hickey, D. L. Marrocco, R. B. Gilchrist, R. J. Norman, and D. T. Armstrong "Interactions Between Androgen and Growth Factors in Granulosa Cell Subtypes of Porcine Antral Follicles," Biology of Reproduction 71(1), 45-52, (1 July 2004). https://doi.org/10.1095/biolreprod.103.026484
Received: 12 December 2003; Accepted: 1 February 2004; Published: 1 July 2004
JOURNAL ARTICLE
8 PAGES


Share
SHARE
KEYWORDS
androgen receptor
cumulus cells
follicular development
granulosa cells
growth factors
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top