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1 November 2004 Gluconeogenesis-Linked Glycogen Metabolism Is Important in the Achievement of In Vitro Capacitation of Dog Spermatozoa in a Medium Without Glucose
J. L. Albarracín, J. M. Fernández-Novell, J. Ballester, M. C. Rauch, A. Quintero-Moreno, A. Peña, T. Mogas, T. Rigau, A. Yañez, J. J. Guinovart, J. C. Slebe, I. I. Concha, J. E. Rodríguez-Gil
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Abstract

In vitro capacitation of dog spermatozoa in a medium without sugars and with lactate as the metabolic substrate (l-CCM) was accompanied by a progressive increase of intracellular glycogen during the first 2 h of incubation, which was followed by a subsequent decrease of glycogen levels after up to 4 h of incubation. Lactate from the medium is the source for the observed glycogen synthesis, as the presence of [14C]glycogen after the addition to l-CCM with [14C]lactate was demonstrated. The existence of functional gluconeogenesis in dog sperm was also sustained by the presence of key enzymes of this metabolic pathway, such as fructose 1,6-bisphophatase and aldolase B. On the other hand, glycogen metabolism from gluconeogenic sources was important in the maintenance of a correct in vitro fertilization after incubation in the l-CCM. This was demonstrated after the addition of phenylacetic acid (PAA) to l-CCM. In the presence of PAA, in vitro capacitation of dog spermatozoa suffered alterations, which translated into changes in capacitation functional markers, like the increase in the percentage of altered acrosomes, a distinct motion pattern, decrease or even disappearance of capacitation-induced tyrosine phosphorylation, and increased heterogeneity of the chlorotetracycline pattern in capacitated cells. Thus, this is the first report indicating the existence of a functional glyconeogenesis in mammalian spermatozoa. Moreover, gluconeogenesis-linked glycogen metabolism seems to be of importance in the maintenance of a correct in vitro capacitation in dog sperm in the absence of hexoses in the medium.

J. L. Albarracín, J. M. Fernández-Novell, J. Ballester, M. C. Rauch, A. Quintero-Moreno, A. Peña, T. Mogas, T. Rigau, A. Yañez, J. J. Guinovart, J. C. Slebe, I. I. Concha, and J. E. Rodríguez-Gil "Gluconeogenesis-Linked Glycogen Metabolism Is Important in the Achievement of In Vitro Capacitation of Dog Spermatozoa in a Medium Without Glucose," Biology of Reproduction 71(5), 1437-1445, (1 November 2004). https://doi.org/10.1095/biolreprod.104.029041
Received: 27 February 2004; Accepted: 1 June 2004; Published: 1 November 2004
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KEYWORDS
dog sperm
gamete biology
Gluconeogenesis
glycogen metabolism
in vitro capacitation
sperm
sperm capacitation
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