Translator Disclaimer
1 November 2004 Composition of Luminal Fluid Secreted by the Seminiferous Tubules and After Reabsorption by the Extratesticular Ducts of the Japanese Quail, Coturnix coturnix japonica
Author Affiliations +
Abstract

The present report examines the composition of luminal fluid in the seminiferous tubule (STF), rete testis (RTF), and ductus epididymidis of the Japanese quail (Coturnix coturnix japonica). This subject is of particular interest, both because the reproductive ducts are intra-abdominal and because sperm production is more rapid in birds than in mammals. It was interpreted that micropuncture samples of STF contain varying amounts of contamination with intracellular solute, particularly K and protein. The concentration of solute in samples was correlated with packed cell volume (spermatocrit), and when the latter was used to assess estimates of solute concentration in STF, the magnitude of the estimates were much the same as determinations in RTF. Consequently, it is concluded that the fluid entering the rete testis of the quail is the primary secretion of the seminiferous tubules. The composition of RTF in the quail was determined to be 148 mM Na, 126 mM Cl, 9.8 mM K, 2.7 mM Mg, 1.4 mM Ca, 2.1 mM glutamate, 3.4 mM glutamine, 20.2 mM bicarbonate, 1.8 μg μl−1 of protein, pH 7.34, and 310 mmol kg−1, and it is significantly different from the composition of blood plasma. Estimates of solute output by the testis and reabsorption by the extratesticular ducts indicate, first, that most of the solutes secreted into the seminiferous tubules are subsequently reabsorbed from the extratesticular ducts and, second, that sufficient solute of testicular origin (except for protein) exists to account for the concentrations of solutes throughout the lumen of the duct system. Changes in the concentration of solute in the extratesticular ducts probably result from different reabsorption rates of solute and water. The composition of fluid from the distal end of the ductus epididymidis was 133 mM Na, 125 mM Cl, 25 mM K, 1.0 mM Mg, 0.3 mM Ca, 6.7 mM glutamate, 4.0 mM glutamine, 19.5 mM bicarbonate, 6.0 μg μl−1 of protein, pH 7.33, and 335 mmol kg−1, and it is significantly different from those of RTF and blood.

J. Clulow and R. C. Jones "Composition of Luminal Fluid Secreted by the Seminiferous Tubules and After Reabsorption by the Extratesticular Ducts of the Japanese Quail, Coturnix coturnix japonica," Biology of Reproduction 71(5), 1508-1516, (1 November 2004). https://doi.org/10.1095/biolreprod.104.031401
Received: 29 April 2004; Accepted: 1 June 2004; Published: 1 November 2004
JOURNAL ARTICLE
9 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top