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1 January 2005 CDC6 Requirement for Spindle Formation During Maturation of Mouse Oocytes
Martin Anger, Paula Stein, Richard M. Schultz
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A master regulator of DNA replication, CDC6 also functions in the DNA-replication checkpoint by preventing DNA rereplication. Cyclin-dependent kinases (CDKs) regulate the amount and localization of CDC6 throughout the cell cycle; CDC6 phosphorylation after DNA replication initiation leads to its proteolysis in yeast or translocation to the cytoplasm in mammals. Overexpression of CDC6 during the late S phase prevents entry into the M phase by activating CHEK1 kinase that then inactivates CDK1/cyclin B, which is essential for the G2/M-phase transition. We analyzed the role of CDC6 during resumption of meiosis in mouse oocytes, which are arrested in the first meiotic prophase with low CDK1/cyclin B activity; this is similar to somatic cells at the G2/M-phase border. Overexpression of CDC6 in mouse oocytes does not prevent resumption of meiosis. The RNA interference-mediated knockdown of CDC6, however, reveals a new and unexpected function for CDC6; namely, it is essential for spindle formation in mouse oocytes.

Martin Anger, Paula Stein, and Richard M. Schultz "CDC6 Requirement for Spindle Formation During Maturation of Mouse Oocytes," Biology of Reproduction 72(1), 188-194, (1 January 2005).
Received: 18 August 2004; Accepted: 1 September 2004; Published: 1 January 2005
gamete biology
oocyte development
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