Transgenic RNA Interference Reveals Role for Mouse Sperm Phospholipase Cζ in Triggering Ca2  Oscillations During Fertilization1

Jason G. Knott; Manabu Kurokawa; Rafael A. Fissore; Richard M. Schultz; Carmen J. Williams

doi:10.1095/biolreprod.104.036244

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1 April 2005 Transgenic RNA Interference Reveals Role for Mouse Sperm Phospholipase Cζ in Triggering Ca² Oscillations During Fertilization

Jason G. Knott, Manabu Kurokawa, Rafael A. Fissore, Richard M. Schultz, Carmen J. Williams

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Biology of Reproduction, 72(4):992-996 (2005). https://doi.org/10.1095/biolreprod.104.036244

Abstract

A sperm-specific phospholipase (PL) C, termed PLCζ, is proposed to be the soluble sperm factor that induces Ca² oscillations in mammalian eggs and, thus, initiates egg activation in vivo. We report that sperm from transgenic mice expressing short hairpin RNAs targeting PLCζ mRNA have reduced amounts of PLCζ protein. Sperm derived from these transgenic mice trigger patterns of Ca² oscillations following fertilization in vitro that terminate prematurely. Consistent with the perturbation in patterns of Ca² oscillations is the finding that mating of transgenic founder males to females results in lower rates of egg activation and no transgenic offspring. These data strongly suggest that PLCζ is the physiological trigger of Ca² oscillations required for activation of development.

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Jason G. Knott, Manabu Kurokawa, Rafael A. Fissore, Richard M. Schultz, and Carmen J. Williams "Transgenic RNA Interference Reveals Role for Mouse Sperm Phospholipase Cζ in Triggering Ca² Oscillations During Fertilization," Biology of Reproduction 72(4), 992-996, (1 April 2005). https://doi.org/10.1095/biolreprod.104.036244

Received: 14 September 2004; Accepted: 1 November 2004; Published: 1 April 2005

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