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1 November 2005 Cumulus Cells Accelerate Aging of Mouse Oocytes
Yi-Liang Miao, Xin-Yong Liu, Tian-Wu Qiao, De-Qiang Miao, Ming-Jiu Luo, Jing-He Tan
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Abstract

The role of cumulus cells (CCs) that surround oocytes in maturation, ovulation, and fertilization has been extensively studied, yet little is known about their role in oocyte aging. Although early studies have shown that when ovulated oocytes are aged in vitro displayed similar morphological alterations as those aged in vivo, a recent study found that vitro culture of mouse oocytes retarded oocyte aging. The objective of this study was to test the hypothesis that CCs would accelerate oocyte aging. During in vitro aging with CCs of both in vivo-matured and in vitro-matured mouse oocytes, activation rates increased, whereas the maturation-promoting factor (MPF) activity decreased significantly as during in vivo aging of the ovulated oocytes. During aging after denudation of CCs, however, activation rates of both in vivo-matured and in vitro-matured oocytes remained low and the MPF activity decreased much more slowly compared to that of oocytes aged with CCs. Although many oocytes aged in vivo and in vitro with CCs showed a partial cortical granule (CG) release, very few cumulus-free oocytes released their CGs during in vitro aging. When denuded oocytes were cultured with cumulus-oocyte-complexes at a 1:2 ratio or on a CC monolayer, activation rates increased, while MPF activity decreased significantly. The results strongly suggested that CCs accelerated the aging progression of both in vivo-matured and in vitro-matured mouse oocytes.

Yi-Liang Miao, Xin-Yong Liu, Tian-Wu Qiao, De-Qiang Miao, Ming-Jiu Luo, and Jing-He Tan "Cumulus Cells Accelerate Aging of Mouse Oocytes," Biology of Reproduction 73(5), 1025-1031, (1 November 2005). https://doi.org/10.1095/biolreprod.105.043703
Received: 10 May 2005; Accepted: 1 June 2005; Published: 1 November 2005
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KEYWORDS
Activation
aging
cumulus cells
gamete biology
mouse oocyte
MPF
oocyte development
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