To increase the manipulation efficiency and storage capability of vitrified human embryonic stem cells, a new bulk vitrification method was established using transformed cryovials. This method vitrified a large number of cell clumps, as opposed to those cryopreserved by a slow-freezing method with conventional cryovials at one time (round). After warming, vitrified human embryonic stem cells exhibited a much higher survival rate than the slow-freezing cells. The vitrified stem cells continued to express markers of pluripotency and formed teratomas in mice with severe combined immunodeficiency, confirming the pluripotency of vitrified-warmed human embryonic stem cell clumps. The new bulk vitrification method is superior to and more practical than the open pulled straw vitrification method and the slow-freezing method for the cryopreservation of human embryonic stem cells.
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14 January 2010
Cryopreservation of Human Embryonic Stem Cells with a New Bulk Vitrification Method
Tao Li,
Qingyun Mai,
Jun Gao,
Canquan Zhou
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Biology of Reproduction
Vol. 82 • No. 5
May 2010
Vol. 82 • No. 5
May 2010
cell strainer
cryopreservation
cryovial
human embryonic stem cells
vitrification