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14 January 2010 Cryopreservation of Human Embryonic Stem Cells with a New Bulk Vitrification Method
Tao Li, Qingyun Mai, Jun Gao, Canquan Zhou
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To increase the manipulation efficiency and storage capability of vitrified human embryonic stem cells, a new bulk vitrification method was established using transformed cryovials. This method vitrified a large number of cell clumps, as opposed to those cryopreserved by a slow-freezing method with conventional cryovials at one time (round). After warming, vitrified human embryonic stem cells exhibited a much higher survival rate than the slow-freezing cells. The vitrified stem cells continued to express markers of pluripotency and formed teratomas in mice with severe combined immunodeficiency, confirming the pluripotency of vitrified-warmed human embryonic stem cell clumps. The new bulk vitrification method is superior to and more practical than the open pulled straw vitrification method and the slow-freezing method for the cryopreservation of human embryonic stem cells.

Tao Li, Qingyun Mai, Jun Gao, and Canquan Zhou "Cryopreservation of Human Embryonic Stem Cells with a New Bulk Vitrification Method," Biology of Reproduction 82(5), 848-853, (14 January 2010).
Received: 14 August 2009; Accepted: 1 January 2010; Published: 14 January 2010
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human embryonic stem cells
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