Polypyrimidine Tract-Binding Protein 2 Binds to Selective, Intronic Messenger RNA and MicroRNA Targets in the Mouse Testis

Mingang Xu; Norman B. Hecht

doi:10.1095/biolreprod.110.087114

We can help you reset your password using the email address linked to your BioOne Complete account.

Registered users receive a variety of benefits including the ability to customize email alerts, create favorite journals list, and save searches. Please note that a BioOne web account does not automatically grant access to full-text content. An institutional or society member subscription is required to view non-Open Access content. Contact helpdesk@bioone.org with any questions.

All Fields are Required

First Name

Last/Family Name

Password

Password Requirements: Minimum 8 characters, must include as least one uppercase, one lowercase letter, and one number or permitted symbol Valid Symbols for password:
~ Tilde
! Exclamation Mark
@ At sign
$ Dollar sign
^ Caret
( Opening Parenthesis
) Closing Parenthesis
_ Underscore
. Period

Confirm Password

Web Account created successfully

Mingang Xu, Norman B. Hecht^1,*

¹Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicin

^*Norman B. Hecht, Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicine, 1310 Biomedical Research Building, 421 Curie Boulevard, Philadelphia, PA 19104-6080. FAX: 215 573 7627; e-mail: nhecht@mail.med.upenn.edu

Abstract

Here we use an in vivo cross-linking and immunoprecipitation procedure to detect RNA targets of the multifunctional RNA-binding protein polypyrimidine tract-binding protein (PTBP) 2 in mouse testis. Eleven known mRNAs, including Ptbp2 mRNA, 28 RNAs matching intron sequences, and 12 small RNAs and repeat sequences are identified. The specificity of interaction between PTBP2 and its target RNAs was confirmed using RNA interference with mouse N2A cells. Reduction of PTBP2 levels led to decreases in 7 of 10 of the mRNAs, to the repression of alternative splicing of introns, and to reductions in specific miRNAs.

Citation Download Citation

Mingang Xu and Norman B. Hecht "Polypyrimidine Tract-Binding Protein 2 Binds to Selective, Intronic Messenger RNA and MicroRNA Targets in the Mouse Testis," Biology of Reproduction 84(3), 435-439, (27 October 2010). https://doi.org/10.1095/biolreprod.110.087114

Received: 6 July 2010; Accepted: 1 October 2010; Published: 27 October 2010

You currently do not have any folders to save your paper to! Create a new folder below.