Humanized mice, which refers to immunodeficient mice repopulated with the human immune system, are powerful tools for study in the field of immunology. It has been difficult, however, to generate these transgenic (Tg) mice directly from such strains as the NOD/SCID mouse. In this study, we describe a method developed by us for the generation of Tg mice on an NOD/SCID background. First, we obtained fertilized eggs efficiently by means of in vitro fertilization (IVF); then, we attempted to generate CAG-EGFP Tg mice on an NOD/SCID background, finding that delayed timing of the microinjection after the IVF improved the time to development of the two-cell-stage embryos and the obtainment of newborns. We successfully generated Tg mice and confirmed the germ-line transmission in the offspring. In conclusion, we established a novel system for directly generating transgenic mice on an NOD/SCID background. This novel system is expected to allow improved efficiency of the generation of humanized mice.
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8 December 2010
Generation of Transgenic Mice on an NOD/SCID Background Using the Conventional Microinjection Technique
Katsuyoshi Kumagai,
Naoto Kubota,
Toshiki I. Saito,
Takayoshi Sasako,
Rumi Takizawa,
Katsuko Sudo,
Mineo Kurokawa,
Takashi Kadowaki
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Biology of Reproduction
Vol. 84 • No. 4
April 2011
Vol. 84 • No. 4
April 2011
embryos
immunodeficient mice
in vitro fertilization
IVF
microinjection
Tg mice