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27 April 2011 Drug-Inducible Gene Recombination by the Dppa3-MER Cre MER Transgene in the Developmental Cycle of the Germ Cell Lineage in Mice
Takayuki Hirota, Hiroshi Ohta, Mayo Shigeta, Hitoshi Niwa, Mitinori Saitou
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Abstract

Germ cells ensure the diversification and totipotency of genetic information via the elaborate genetic and epigenetic regulation of the genome architecture during their development. To understand the mechanism underlying the regulation of genome function in germ cells, it is of primary importance to develop systems in which gene function can be regulated at desired time points during their development. Here, we report the generation of transgenic strains that express Cre recombinase flanked by the ligand-binding domains of murine estrogen receptor (MER Cre MER [MCM]) under the control of the regulatory elements of the Dppa3 (also known as Stella or Pgc7) gene. On the administration of 4-hydroxytamoxifen (4-OHT), the Dppa3-MCM strains recombined the sequence flanked by the loxP elements (the floxed sequence) specifically in primordial germ cells as early as Embryonic Day (E) 7.0, and this recombination became robust after E9.5. Furthermore, these strains exhibited efficient and specific recombination of the floxed sequence during the growth of oocytes and in preimplantation embryos in the 4-OHT-dependent manner. Thus, these Dppa3-MCM strains offer valuable opportunities to explore gene function in both loss-of-function and gain-of-function experiments at a variety of time points during germ cell development.

Takayuki Hirota, Hiroshi Ohta, Mayo Shigeta, Hitoshi Niwa, and Mitinori Saitou "Drug-Inducible Gene Recombination by the Dppa3-MER Cre MER Transgene in the Developmental Cycle of the Germ Cell Lineage in Mice," Biology of Reproduction 85(2), 367-377, (27 April 2011). https://doi.org/10.1095/biolreprod.110.090662
Received: 28 December 2010; Accepted: 1 April 2011; Published: 27 April 2011
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KEYWORDS
early development
Genetics
oocyte
primordial germ cells
transgenic/knockout model
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