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20 March 2013 Cyclin O Regulates Germinal Vesicle Breakdown in Mouse Oocytes
Jun-Yu Ma, Ying-Chun Ou-Yang, Yi-Bo Luo, Zhen-Bo Wang, Yi Hou, Zhi-Ming Han, Zhonghua Liu, Heide Schatten, Qing-Yuan Sun
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Abstract

It is well accepted that oocyte meiotic resumption is mainly regulated by the maturation-promoting factor (MPF), which is composed of cyclin B1 (CCNB1) and cyclin-dependent kinase 1 (CDC2). Maturation-promoting factor activity is regulated by the expression level of CCNB1, phosphorylation of CDC2, and their germinal vesicle (GV) localization. In addition to CCNB1, cyclin O (CCNO) is highly expressed in oocytes, but its biological functions are still not clear. By employing short interfering RNA microinjection of GV-stage oocytes, we found that Ccno knockdown inhibited CDC2 (Tyr15) dephosphorylation and arrested oocytes at the GV stage. To rescue meiotic resumption, cell division cycle 25 B kinase (Cdc25b) and Ccnb1 were overexpressed in the Ccno knockdown oocytes. Unexpectedly, we found that Ccno knockdown did not affect CDC25B entry into the GV, and overexpression of CDC25B was not able to rescue resumption of oocyte meiosis. However, GV breakdown (GVBD) was significantly increased after overexpression of Ccnb1 in Ccno knockdown oocytes, indicating that GVBD block caused by cyclin O knockdown can be rescued by cyclin B1 overexpression. We thus conclude that cyclin O, as an upstream regulator of MPF, plays an important role in oocyte meiotic resumption in mouse oocytes.

Jun-Yu Ma, Ying-Chun Ou-Yang, Yi-Bo Luo, Zhen-Bo Wang, Yi Hou, Zhi-Ming Han, Zhonghua Liu, Heide Schatten, and Qing-Yuan Sun "Cyclin O Regulates Germinal Vesicle Breakdown in Mouse Oocytes," Biology of Reproduction 88(5), (20 March 2013). https://doi.org/10.1095/biolreprod.112.103374
Received: 12 July 2012; Accepted: 1 March 2013; Published: 20 March 2013
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KEYWORDS
Ccnb1
CDC25B
CDC25B
cyclin B1
cyclin O
meiosis arrest
oocyte maturation
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