Translator Disclaimer
1 May 2013 Minimal Concentrations of Retinoic Acid Induce Stimulation by Retinoic Acid 8 and Promote Entry into Meiosis in Isolated Pregonadal and Gonadal Mouse Primordial Germ Cells
Marianna Tedesco, Maria Giovanna Desimio, Francesca Gioia Klinger, Massimo De Felici, Donatella Farini
Author Affiliations +
Abstract

In the present study, we demonstrate that minimal concentrations (≤1 nM) of retinoic acid (RA), equivalent to the quantity contaminating serum-containing culture medium, are sufficient to promote meiotic entry and progression through meiotic prophase I (MPI) stages in isolated 12.5-days postcoitum (dpc) XX and XY mouse primordial germ cells (PGCs) in culture. Similarly, we found that the same low RA concentration up-regulated or induced stimulation by retinoic acid 8 (Stra8) in such cells, both at mRNA and protein level. In preleptotene/leptotene germ cells, STRA8 was localized in nuclear dots that disappeared at later MPI stages. In addition to Stra8, other meiotic genes such as Dmc1 and Rec8 appeared stimulated by RA directly in PGCs with similar concentration-dependent trends. Finally, we found that RA induced Stra8, Sycp3, Dmc1, and Rec8 transcripts, promoting meiotic entry in culture also in pregonadal 10.5-dpc PGCs of both sexes. When cultured isolated from somatic cells, such PGCs, however, were unable to progress through MPI stages, while after entering meiosis, they progressed through MPI when cultured within aorta/gonad/mesonephros tissues. We conclude that besides RA, germ cell intrinsic factors and other exogenous signals from the surrounding somatic cells are probably necessary for meiotic entry and progression in mouse PGCs.

Marianna Tedesco, Maria Giovanna Desimio, Francesca Gioia Klinger, Massimo De Felici, and Donatella Farini "Minimal Concentrations of Retinoic Acid Induce Stimulation by Retinoic Acid 8 and Promote Entry into Meiosis in Isolated Pregonadal and Gonadal Mouse Primordial Germ Cells," Biology of Reproduction 88(6), (1 May 2013). https://doi.org/10.1095/biolreprod.112.106526
Received: 27 November 2012; Accepted: 1 April 2013; Published: 1 May 2013
JOURNAL ARTICLE
PAGES


Share
SHARE
KEYWORDS
meiosis
primordial germ cells
retinoic acid
Stra8
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top