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17 April 2013 t-SNARE Syntaxin2 (STX2) Is Implicated in Intracellular Transport of Sulfoglycolipids During Meiotic Prophase in Mouse Spermatogenesis
Yasuhiro Fujiwara, Narumi Ogonuki, Kimiko Inoue, Atsuo Ogura, Mary Ann Handel, Junko Noguchi, Tetsuo Kunieda
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Abstract

Syntaxin2 (STX2), also known as epimorphin, is a member of the SNARE family of proteins, with expression in various types of cells. We previously identified an ENU-induced mutation, repro34, in the mouse Stx2 gene. The Stx2repro34 mutation causes male-restricted infertility due to syncytial multinucleation of spermatogenic cells during meiotic prophase. A similar phenotype is also observed in mice with targeted inactivation of Stx2, as well as in mice lacking enzymes involved in sulfoglycolipid synthesis. Herein we analyzed expression and subcellular localization of STX2 and sulfoglycolipids in spermatogenesis. The STX2 protein localizes to the cytoplasm of germ cells at the late pachytene stage. It is found in a distinct subcellular pattern, presumably in the Golgi apparatus of pachytene/diplotene spermatocytes. Sulfoglycolipids are produced in the Golgi apparatus and transported to the plasma membrane. In Stx2repro34 mutants, sulfoglycolipids are aberrantly localized in both pachytene/diplotene spermatocytes and in multinucleated germ cells. These results suggest that STX2 plays roles in transport and/or subcellular distribution of sulfoglycolipids. STX2 function in the Golgi apparatus and sulfoglycolipids may be essential for maintenance of the constriction between neighboring developing spermatocytes, which ensures ultimate individualization of germ cells in later stages of spermatogenesis.

Yasuhiro Fujiwara, Narumi Ogonuki, Kimiko Inoue, Atsuo Ogura, Mary Ann Handel, Junko Noguchi, and Tetsuo Kunieda "t-SNARE Syntaxin2 (STX2) Is Implicated in Intracellular Transport of Sulfoglycolipids During Meiotic Prophase in Mouse Spermatogenesis," Biology of Reproduction 88(6), (17 April 2013). https://doi.org/10.1095/biolreprod.112.107110
Received: 18 December 2012; Accepted: 1 April 2013; Published: 17 April 2013
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KEYWORDS
glycolipids
meiosis
Mouse
spermatogenesis
Stx2
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