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2 April 2014 Comprehensive Analysis of Prostaglandin Metabolic Enzyme Expression During Pregnancy and the Characterization of AKR1B1 as a Prostaglandin F Synthase at the Maternal-Conceptus Interface in Pigs
Heewon Seo, Yohan Choi, Jangsoo Shim, Inkyu Yoo, Hakhyun Ka
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Abstract

Prostaglandins (PGs) are important lipid mediators regulating various reproductive processes in many species. In pigs, the expression pattern of PGE2 and PGF metabolic enzymes and the regulatory mechanism controlling PGE2 and PGF levels in the uterus during pregnancy are not completely understood. This study determined endometrial expression of the genes (PLA2G4A, PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, CBR1, and HPGD) involved in PGE2 and PGF metabolism during the estrous cycle and pregnancy and measured levels of PGE2 and PGF in uterine endometrial tissues and uterine flushings at the time of conceptus implantation in pigs. Except PTGES3, expression of the genes studied changed in a pregnancy-stage-specific manner, and localization of PTGES, AKR1B1, CBR1, and HPGD mRNAs were cell-type specific in the uterine endometrium. Levels of both PGE2 and PGF in uterine endometrial tissues and uterine lumen were higher on Day 12 of pregnancy than those of the estrous cycle and affected by different morphology of spherical and filamentous conceptuses. Furthermore, we determined that endometrial expression of AKR1B1, known to encode a PGF synthase in other species, was increased by estrogen and interleukin-1beta and that AKR1B1 exhibited PGF synthase activity in the porcine uterine endometrium. These results in pigs indicate that the PGE2 and PGF metabolic enzymes are expressed stage specifically in the endometrium during pregnancy and regulate the abundance of PGE2 and PGF in the uterus at the time of implantation and that AKR1B1 may act as a major PGF synthase in the endometrium during early pregnancy.

Heewon Seo, Yohan Choi, Jangsoo Shim, Inkyu Yoo, and Hakhyun Ka "Comprehensive Analysis of Prostaglandin Metabolic Enzyme Expression During Pregnancy and the Characterization of AKR1B1 as a Prostaglandin F Synthase at the Maternal-Conceptus Interface in Pigs," Biology of Reproduction 90(5), (2 April 2014). https://doi.org/10.1095/biolreprod.113.114926
Received: 13 October 2013; Accepted: 1 March 2014; Published: 2 April 2014
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