Acquisition of hospital and community-associated methicillin resistant Staphylococcus aureus (MRSA), extended spectrum beta lactamase producing Escherichia coli (ESBL-E. coli) and carbapenemase-producing Klebsiella pneumoniae (CPKP) from dirty environments is widely reported in Nigeria. Restroom floors of congested hostels (dormitories) and high-touch surfaces such as corridors, doors, and wardrobes handles, and hands of restroom users and non-users were studied for the presence of MRSA, ESBL-E. coli and CPKP before and 1 hour after cleaning and disinfection. Surfaces were swabbed with sterile moistened swab sticks, and isolation and identification of S. aureus, E. coli and K. pneumoniae were performed using standard techniques. Methicillin resistance by S. aureus, ESBL production by E. coli and carbapenemase production by K. pneumoniae were determined phenotypically using cefoxitin susceptibility, double disc synergy and modified Hodges tests, respectively. Amplification of bla_CTX-M, and bla_NDM-1 in ESBL-E. coli and CPKP was performed using PCR. We found that restroom floors were frequently contaminated with MRSA, ESBL-E. coli, and CPKP before and 1 hour after cleaning. High-touch surfaces such as corridors, doors, and wardrobes handles were contaminated with these pathogens, as well as hands of restroom users. The process of floor disinfection was able to reduce the percentage of MRSA, ESBL-E. coli and CPKP by 23.8%, 50%, and 22.2%, respectively. Pathogens from restroom floors and hand of users harbor similar resistant genes. The findings suggest that people with frequent contact with these surfaces can acquire and disseminate the pathogens to others.