Translator Disclaimer
17 December 2019 Structural features and antiviral function of the MDA5 gene in ducks (Anas platyrhynchos)
Author Affiliations +
Abstract

Melanoma differentiation-associated gene 5 (MDA5) is an important cytoplasmic RNA sensor that detects viral double-stranded RNA in innate immunity. The objective of this study was to characterize the structure and function of the MDA5 gene in the duck. In this study, full-length duck MDA5 (duMDA5) complementary DNA (cDNA) was obtained using the reverse transcription-polymerase chain reaction and rapid amplification of the cDNA ends. The cDNA consisted of a 123 nucleotide 5′ untranslated region (UTR), a 735 nucleotide 3′ UTR, and a 3012 nucleotide open-reading frame, encoding 1003 amino acids. Multiple sequence alignments showed that duMDA5 had 91.18% and 83.11% amino acid sequence similarity with geese and chicken MDA5, respectively, as well as 59.76%–61.26% sequence identity with mammalian homologs. Phylogenetic analysis demonstrated that MDA5 has been highly conserved throughout vertebrate evolution. Quantitative real-time polymerase chain reaction analysis indicated that the duMDA5 mRNA is scarcely detected in healthy tissues and the highest relative transcript level of duMDA5 was induced during poly(I:C) stimulation. Furthermore, knockdown duMDA5 significantly inhibited the transcription of poly(I:C)-induced beta interferons, nuclear factor kappa-B, interferon regulatory factor 7, translocated intimin receptor domain-containing adaptor protein inducing beta interferons, interferon-induced GTP-binding protein, signal transducer and activator of transcription 1 and 2 mRNA. Taken together, these results suggest that duMDA5 is an important receptor for inducing antiviral activity in the duck’s innate immune response.

Copyright remains with the author(s) or their institution(s). Permission for reuse (free in most cases) can be obtained from copyright.com.
Tiantian Gu, Guoqin Li, Yong Tian, Li Chen, Xinsheng Wu, Tao Zeng, Qi Xu, Spyrydonov Vladyslav, Guohong Chen, and Lizhi Lu "Structural features and antiviral function of the MDA5 gene in ducks (Anas platyrhynchos)," Canadian Journal of Animal Science 100(2), 359-367, (17 December 2019). https://doi.org/10.1139/cjas-2019-0161
Received: 2 September 2019; Accepted: 30 November 2019; Published: 17 December 2019
JOURNAL ARTICLE
9 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top