Sixty-nine finishing steers were randomly assigned to one of two treatment groups: supplementation to provide 0 or 267 mg·d⁻¹ of ractopamine hydrochloride (RAC) for 42 d. Twelve steers were slaughtered for tissue and blood collection. Final body weight (BW) tended to be greater with RAC supplementation. Ractopamine hydrochloride supplementation decreased liver mass as a percentage of BW. Pancreatic protein concentration, digestive enzyme activities, serum glucose, and insulin concentrations were not influenced by RAC supplementation. Ractopamine hydrochloride supplementation decreased the pancreatic:α-amylase:trypsin and serum urea nitrogen concentrations. These data suggest that RAC may influence pancreatic exocrine function in cattle.