Acosta, D. A. V., Pfeifer, L. F. M., Schmitt, E., Schneider, A., Silveira, P. A. S., Jacometo, C. B., Brauner, C. C., Rabassa, V. R., Corrêa, M. N. and Del Pino, F. A. B. 2013. Effect of prepartum somatotropin injection in late pregnant Holstein heifers with high body condition score on metabolic parameters, resumption of ovulation and milk production. Can. J. Anim. Sci. 93: 287-292. In the early post-partum period of dairy cows the duration and intensity of negative energy balance, the level of body condition score (BCS) loss and the milk yield are strongly associated with the timing of the first ovulation. The aim of this study was to determine the effect of pre-partum injections of somatotropin in dairy heifers with high BCS on the metabolism, resumption of ovarian activity and milk production. Holstein heifers (n=20) with high BCS, were divided randomly into two groups: somatotropin (n=10), which received two doses of somatotropin (500 mg) at -28 and -14 d from calving and Control (n=10), which received placebo. Blood samples were collected for evaluation of ß-hydroxybutyrate (BHBA) and non-esterified fatty acids (NEFA) concentrations. Follicular development was also monitored via ultrasound. Somatotropin had no effect on plasma NEFA (P=0.35 and P=0.46) or BHBA (P=0.20 and P=0.44,) concentrations in the pre-partum and post-partum period, respectively. Milk production was not different between control (17.53±0.66 kg cow^-1 d^-1) and somatotropin groups (16.13±0.67 kg cow^-1 d^-1) (P=0.14). Pre-partum somatotropin administration did not affect (P=0.28) the time of the first post-partum ovulation. The proportion of cows ovulating the first post-partum follicular wave was not different between groups (P=0.49). In conclusion, pre-partum somatotropin treatment in dairy heifers with high body condition score seems not to have any effect on markers of energy balance, milk production or development of the first follicular wave in the early post-partum period.