The aim of the study was to determine the number and shape of nucleoli during meiosis in cells of the domestic horse. In addition, the level of global DNA methylation was determined using a quantitative technique for measuring the relative level of DNA methylation, modelled on an immunoenzymatic assay. The research was carried out on stallions belonging to two age groups (2 and 7 yr). In the cells of the 2-yr-old animals, the nucleoli were mainly of a regular shape and no fragmented nucleoli were observed. The cells of the 7-yr-old horses had a small percentage of regularly shaped nucleoli, and nucleoli with a fragmented structure were present. The study provides a basis for further research on epigenetic mechanisms in horses.
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Vol. 96 • No. 2