Martens, G., Lamari, L., Grieger, A., Gulden, R. H. and McCallum, B. 2014. Comparative yield, disease resistance and response to fungicide for forty-five historic Canadian wheat cultivars. Can. J. Plant Sci. 94: 371-381. Forty-five historic Canadian spring wheat cultivars, ranging from Red Fife (1870) to modern cultivars, were compared for yield and disease resistance in field trials from 2007 to 2010. A split-plot design was used to test yield, leaf rust and Fusarium head blight resistance, with or without a fungicide application. Older cultivars were generally lower yielding and more leaf rust susceptible than modern cultivars; this difference was greatest in 2007 and 2010 under heavier leaf rust. Response to fungicide application was highest in 2007 and 2010. In 2008, leaf rust was very low, and fungicide application had a slightly negative effect on yield overall. Cultivars that have good leaf rust resistance, such as Pasqua, AC Minto, and 5600 HR, had a negligible response to fungicide, whereas older, susceptible cultivars had a larger response. Fusarium head blight levels were too low to compare the cultivars. The highest-yielding cultivars in the untreated plots were AC Domain, 5500HR, AC Cora, Roblin and Barrie, whereas AC Cora, AC Domain, McKenzie, Roblin and AC Intrepid were the highest yielding in the fungicide-treated plots. AC Domain, AC Barrie and Roblin were among the most popular wheat cultivars in Manitoba, in terms of seeded area from the early 1990s to 2009.