Cherry leaf spot (CLS) disease caused by Blumeriella jaapii is a significant constraint in nursery production of flowering cherries in the southeastern United States. The objectives of this study were to evaluate six commercial cultivars for host resistance to CLS and identify effective fungicides that can be recommended to growers. Out of six cultivars ‘Kwanzan’, ‘Okame’, ‘Autamnalis’, ‘Snowgoose’, ‘Yoshino’, and ‘Akebono’ evaluated for host resistance, only ‘Kwanzan’ displayed moderate resistance with the least amount of disease symptoms in a shade-house environment, followed by ‘Autamnalis’. ‘Yoshino’ was most susceptible to CLS in both the shade-house and field experiments. Out of seven fungicides evaluated individually and in rotations, captan in rotations with tebuconazole and trifloxystrobin and captan in rotations with chlorothalonil and acibenzolar-S-methyl were most effective in controlling CLS. A biopesticide (neem seed oil extract) and a biological control isolate (Stenotrophomonas spp.) were also effective in controlling CLS either alone or in rotation with conventional fungicides. This study identified effective fungicides, a biopesticide, and a biological agent that can be used along with resistant cultivars in CLS disease management.