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12 September 2017 A MADS-box gene associated with protocorm-like body formation in Rosa canina alters floral organ development in Arabidopsis
Kedong Xu, Kun Liu, Jianxin Wu, Wei Wang, Yuling Zhu, Chunjing Li, Mengzhu Zhao, Youwei Wang, Chengwei Li, Liangjun Zhao
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Abstract

We isolated a gene encoding a putative MADS-box protein designated RcAGL15 from a protocorm-like body (PLB), a somatic embryogenetic structure) of Rosa canina L. RcAGL15 encodes a predicted protein of 251 amino acid residues with a molecular weight of 28 kDa and a theoretical isoelectric point of 7.5. Reverse-transcription polymerase chain reaction shows that RcAGL15 is mainly expressed in PLB and regenerated PLB, but not in leaves of aseptic seedlings, incubated leaflets before callus formation, calli, rhizoids, or leaves of regenerated shoots. Transgenic, ectopic expression of RcAGL15 in Arabidopsis changes numbers of floral organs and reduces content of indole-3-acetic acid, but increases levels of abscisic acid. Thus, RcAGL15 appears to be a new MADS-box gene that plays regulatory roles in morphological development and the number of flower organs, as well as somatic embryogenesis.

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Kedong Xu, Kun Liu, Jianxin Wu, Wei Wang, Yuling Zhu, Chunjing Li, Mengzhu Zhao, Youwei Wang, Chengwei Li, and Liangjun Zhao "A MADS-box gene associated with protocorm-like body formation in Rosa canina alters floral organ development in Arabidopsis," Canadian Journal of Plant Science 98(2), 309-317, (12 September 2017). https://doi.org/10.1139/cjps-2017-0027
Received: 22 January 2017; Accepted: 26 July 2017; Published: 12 September 2017
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