A rapid bioassay to screen soils for toxicity of residual petroleum hydrocarbons

John Ashworth; Ian Bullecer

doi:10.1139/CJSS2012-009

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John Ashworth,¹ Ian Bullecer¹

¹ALS Environmental Group, 9936-67 Ave., Edmonton, Alberta, Canada T6E 0P5

Abstract

Ashworth, J. and Bullecer, I. 2012. A rapid bioassay to screen soils for toxicity of residual petroleum hydrocarbons. Can. J. Soil. Sci. 92: 901-904. The following 2-h procedure is proposed as a screen to indicate how soils contaminated with petroleum hydrocarbons (PHC) might perform in bioassays required for site remediation in Canada. Bio-available PHC in soil is extracted by aqueous cyclodextrin; a centrifuged extract portion is treated with amylase, then subjected to a standard Microtox^® bioassay. Extract toxicity is correlated to available PHC content.

Citation Download Citation

John Ashworth and Ian Bullecer "A rapid bioassay to screen soils for toxicity of residual petroleum hydrocarbons," Canadian Journal of Soil Science 92(6), 901-904, (1 November 2012). https://doi.org/10.1139/CJSS2012-009

Received: 31 January 2012; Accepted: 22 August 2012; Published: 1 November 2012