The goal of this study was to test the effectiveness of analyzing sex hormone profiles to sex green sea turtle (Chelonia mydas) hatchlings. To assess the efficacy of this method, 200 eggs of C. mydas were incubated at five different temperatures, and a chemiluminescent immunoassay system was used to determine the sex hormone profiles (estrogen and testosterone) in the amniotic fluid (from eggshells) and plasma of a subset of C. mydas hatchlings. Results were compared to a standard histological technique and revealed that evaluating sex hormone profiles from amniotic fluid is an effective, noninvasive technique for determining hatchling gender.
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1 July 2011
Evaluating Noninvasive Methods of Sex Identification in Green Sea Turtle (Chelonia mydas) Hatchlings
Zhong-Rong Xia,
Pi-Peng Li,
He-Xiang Gu,
Jonathan J. Fong,
Er-Mi Zhao
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Chelonian Conservation and Biology
Vol. 10 • No. 1
July 2011
Vol. 10 • No. 1
July 2011
estradiol
Guangdong Province
Sea Turtle Bay
temperature sex determination
testosterone