Nineteen adult Cajun chorus frogs, Pseudacris fouquettei, were collected in Union County, Arkansas, U.S.A. (n  =  14), and Bowie County, Texas, U.S.A. (n  =  5), and examined for parasites and symbiotic protozoa. All 19 frogs were infected with 1 or more species, including 18 (95%) with Opalina sp., 15 (79%) with Nyctotherus cordiformis, 6 (32%) with Myxidium melleni, 1 (5%) with Mesocoelium monas, 1 (5%) with an unidentified acuariid larva, 1 (5%) with Physaloptera sp. (third stage larva), 3 (16%) with Oswaldocruzia leidyi, and 2 (11%) with Cosmocercoides variabilis. Multiple infections with both protists and helminths were observed in 5 (26%) of the P. fouquettei. We document new host records for Opalina sp., N. cordiformis, Me. monas, Acuariidae gen. sp., Physaloptera sp., and O. leidyi; new distributional records are reported for My. melleni (Arkansas) and O. leidyi (Arkansas and Texas).