Two individual physid snails that were collected from the Middle Rio Grande in New Mexico in August 2007 each released a distinct type of digenean cercaria. The cercariae were photographed, and DNA was extracted from both types of cercariae and their snail hosts. Nuclear 18S and 28S rDNA gene sequences of both digeneans and mitochondrial 16S rDNA sequences for the snails were PCR amplified and sequenced directly. Snails were identified as Physella acuta based on sequence data. Phylogenetic analyses relative to a preexisting set of concatenated 18S and 28S sequences from various digeneans, and their associations with P. acuta as snail host, indicated that the 2 parasites represent an unclassified spirorchid and a paramphistome, respectively. The data provided may aid in future resolution of the complete lifecycles of these digenean parasites and in the evaluation of their impact on the fauna in the unique Rio Grande Bosque region of New Mexico.
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Vol. 81 • No. 1