Thirty-six Brook Silversides, Labidesthes sicculus, and 15 Golden Silversides, Labidesthes vanhyningi, collected from the Ouachita and Red River drainage basins of Arkansas and Oklahoma, U.S.A., were examined for parasites. Six (17%) of the L. sicculus and 3 (20%) of the L. vanhyningi were infected with Dermocystidium sp., 1 (7%) L. vanhyningi harbored an immature tapeworm, Proteocephalus sp., and 1 (7%) L. vanhyningi was infected with the copepod Ergasilus funduli. We document new host records for all 3 parasites as well as 2 new state records for Dermocystidium sp. and E. funduli.