An efficient regeneration and transformation system was established and optimised for adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi). 6-Benzylaminopurine at 5 mg L–1 was used to increase adventitious bud induction frequency. The highest frequency of shoot elongation was 92.8% when using a medium composition of MS salts combined with 0.1 mg L–1 of IAA, 0.5 mg L–1 of GA3, 1.0 mg L–1 of zeatin-riboside, 50 mg L–1 of aspartic acid, and 50 mg L–1 of glutamic acid. In vitro rooting was 100% when shoots were cultured on the solid MS medium supplemented with 1.0 mg L–1 of NAA. Reproducible transformation of epicotyl explants was developed using the A. tumefaciens EHA105 strain. Using a concentration of 40 mg L–1 of acetosyringone, 20 mm MES, and 5 mg L–1 of 6-benzylaminopurine in the co-cultivation medium, a transformation efficiency of 12.6% was attained. Using this transformation protocol, we obtained transgenic adzuki bean plants resistant to soybean mosaic virus by introducing the V. angularis VaPR3 gene.
How to translate text using browser tools
27 February 2013
Disease-resistant transgenic adzuki bean plants obtained through an efficient transformation system
Huatao Chen,
Xin Chen,
Heping Gu,
Xingxing Yuan,
Hongmei Zhang,
Xiaoyan Cui
ACCESS THE FULL ARTICLE

Crop and Pasture Science
Vol. 63 • No. 11–12
March 2013
Vol. 63 • No. 11–12
March 2013
co-cultivation
epicotyls
regeneration
Vigna angularis