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13 December 2018 Molecular characterisation of the NAM-1 genes in bread wheat in Australia
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Abstract

The wheat NAM-B1 and NAM-A1 genes are positively associated with grain protein content (GPC) in wheat. We conducted molecular characterisation of the NAM-1 genes in 51 Australian wheat varieties (Triticum aestivum L.), with the aim of improving GPC and nitrogen-usage efficiency in Australian wheat. In summary, the wild type NAM-B1 gene, which originated from Israel, was identified in two Australian wheat varieties. Five varieties contained a deletion allele, whereas the majority (43) harboured a non-functional NAM-B1 allele and one variety contained both functional and non-functional alleles. Twenty-six Australian wheat varieties contained the NAM-A1a haplotype, which was similar to its well-characterised homoeolog NAM-B1 wild type and associated with high GPC. The NAM-D1 gene in the 51 wheat varieties was also characterised, and no gene variation in the exon regions was noted; only two single-nucleotide polymorphisms in introns 1 and 2 were found among the 51 varieties.

© CSIRO 2018
Rongchang Yang, Angela Juhasz, Yujuan Zhang, Xueyan Chen, Yinjun Zhang, Maoyun She, Jingjuan Zhang, Rowan Maddern, Ian Edwards, Dean Diepeveen, Shahidul Islam, and Wujun Ma "Molecular characterisation of the NAM-1 genes in bread wheat in Australia," Crop and Pasture Science 69(12), 1173-1181, (13 December 2018). https://doi.org/10.1071/CP18273
Received: 13 June 2018; Accepted: 4 October 2018; Published: 13 December 2018
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