Translator Disclaimer
1 August 2017 Eighteen SSR Markers for the Japanese Clawed Salamander, Onychodactylus japonicus, and Cross-Amplification in its Congeners
Natsuhiko Yoshikawa, Nobuaki Nagata
Author Affiliations +
Abstract

We developed 18 novel microsatellite markers for Onychodactylus japonicus using Illumina Miseq System, and tried cross-amplifications in three congeners. The number of alleles and expected heterozygosity at each locus in O. japonicus were 3–15 (mean=6.83) and 0.45–0.89 (mean=0.73), respectively. Cross-amplifications in congeners succeeded in 9–13 of 18 loci, and eight loci were consistently amplified across all four species. The markers described here will be useful not only for investigating phylogeography and fine-scale genetic structure, but also for estimating genetic diversity of endangered species or populations for conservation management.

© 2017 by The Herpetological Society of Japan
Natsuhiko Yoshikawa and Nobuaki Nagata "Eighteen SSR Markers for the Japanese Clawed Salamander, Onychodactylus japonicus, and Cross-Amplification in its Congeners," Current Herpetology 36(2), 153-158, (1 August 2017). https://doi.org/10.5358/hsj.36.153
Accepted: 1 June 2017; Published: 1 August 2017
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top