Marwa S. Salama, Thanaa M. Sileem, Reda S. Hassan, Waheed A. A. Sayed
Entomological News 131 (5), 254-264, (6 November 2024) https://doi.org/10.3157/021.131.0502
KEYWORDS: Galleria mellonella, diet ingredients, date seed, paraffin wax, Quality control
Galleria mellonella (L.) is a worldwide major devastating insect pest of honey bee colonies and causes significant damage in the stored beekeeping industry. Mass rearing of this pest is critical where it considered a valuable and a favorable alternative invertebrate model for studies in biology, behavior, ecology, molecular biology, physiology, and control. For this purpose, newly hatched larvae were reared on five different nutritional diets until reaching the full grown 6th instar to evaluate their fitness and quality control parameters. The possibility to reduce insect rearing costs were assessed by replacing skimmed milk powder (added for its protein and energy intake) with ground date seed and replace glycerin with Paraffin wax as alternative and cheaper sources. The results indicated that the pupation percentage recorded 98, 96, 94, 100, and 100% for G. mellonella reared on diets D1, D2, D3, D4, and D5, respectively. Also, survival percentage was unaffected significantly by diet type, it was, 94, 94, 90, 98, and 96% respectively. Larvae fed on D4 and D5 diets recorded an equal value of growth rate which recorded 10.1 mg/day approximately, however, the lowest growth rate was (8.8 mg/day) for D1 (standard diet). After irradiation with 150 Gy, the average number of eggs laid by irradiated females crossed with normal males was 1134.4 and 1124.6 eggs/female for D5 and D1, respectively. Additionally, the average number of eggs laid when irradiated males crossed with normal females was 1278 and 1293 eggs/female for D1 and D5; respectively. Our findings suggest that the lowest cost of alternative artificial diets could be used for G. mellonella rearing protocol by replacing the skimmed milk powder and Paraffin wax with the ground date seed and glycerin.